ninhydrin

longa [5,6] Chemical Method Result substance tested Alkaloid Mayer's test + Dragendorff's test + Hagers test + Wagner's test + Tannins present Ferric chloride test + Gelatin test + Lead acetate test + Flavonoids Ferric chloride test + Magnesium hydrochloride test + Sodium hydroxide test + Mineral acid test + Carbohydrates Molisch's test + Benedict's test + Fehling's test + Barfoed's test - Phytosterols Liebermann-burchard test + Proteins and amino Millions test - acids Biuret test - Ninhydrin test - Lactones Acetic anhydride test + Alkaline nitroprusside test + Triterpenes Salkowski test + Liebermann-storch mora sky + test Hirshhorn test + Anthraquinone Borntrager's test + glycosides C.

Ninhydrin test: To the extract, 0.25% Ninhydrin reagent was added and boiled for few a minutes.

The ninhydrin test was used to visualize feeding as described by Kanrar et al.

Different plant Tests constituents Test for alkaloids Mayer's test, Dragendroff's test, Wagner's test, and Hager's test Test for proteins and Millon's test, Biuret test, and amino acids ninhydrin test Test for anthraquinones Modified Borntrager's test Test for flavonoids Ferric chloride test and lead acetate test Test for carbohydrates Molisch's test Test for reducing sugars Fehling's test and Benedict's test Test for saponins Foam formation test Test for tannins Ferric chloride test and lead acetate test Test for steroids, Liebermann-Burchard test, triterpenoids, and Salkowski test, Noller's test, cardiac glycosides Legal's test, Baljet test, and Keller Kiliani's test TABLE 2: Effect on electrolyte excretion in urine.

It responded positively to Ninhydrin test. The ESIMS exhibited molecular ion peak [M+H]+ at m/z 148 corresponding to molecular formula [C.sub.6][H.sub.13]N[O.sub.3].