The SNAN fractions (free amino acid, peptide and soluble protein) in RD and OD collected at 2-h intervals were assessed by
ninhydrin assay.
Developing with
ninhydrin (acetone base): The reagent was applied to the sample using a spray, then allowed to dry.
In addition, this method is more time-consuming than the
ninhydrin test and requires the use of relatively complex and expensive equipment (e.g., filter fluorometer and TLC scanner).
Amino N was determined using
ninhydrin with glycine as a standard (Rosen, 1957).
Ninhydrin is used to develop latent prints on porous surfaces.
Proline assay: Three pea plants from each breeding lines were carry outto determine theprolinecontent.To determine the proline content of leaves the acid
ninhydrin method was used (Bates et al., 1973).
The analyte and aqueous
ninhydrin solution upon heating for 2-5 min produced the Ruhemann purple colored drug-derivative which was detected at two wavelengths, 400 nm and 567 nm.
After removal of the HCl by evaporation under vacuum, the amino acids were separated by ion-exchange chromatography on a Shimadzu RF-10AXL sodium column and detected following postcolumn derivatization with
ninhydrin, by measuring absorbance at 350-450 nm.
Amino acid concentration in the leaf was determined by the
Ninhydrin method [33] using L-leucine as the standard.
An improved calorimetric determination of amino acids with the use of
ninhydrin. Anal.
Several methods for the diagnosis of GAMT deficiency by determination of GAA concentrations in biological fluids have been reported (14,17-21), including methods based on liquid chromatography with postcolumn derivatization with
ninhydrin (14, 21), gas chromatography-mass spectrometry (18,19), and tandem mass spectrometry (20).