In November 2017, the patient underwent skin biopsy that showed leukocytoclastic vasculitis and negative staining for
Congo red and [kappa]-light chain (Figure 2).
Detection of biofilm formation by
Congo red agar (CRA)
Following previously described method,18 the test organisms were streaked on CRA which comprised blood-base agar supplemented with glucose and
Congo red dye.
(1) Because our patient's biopsies were consistently negative for
Congo red stain, it was important to have a hematology/oncology evaluation to rule out other causes of abnormal protein deposition, such as multiple myeloma.
Congo red staining of amyloid under light microscopy with polarized light classically produces the appearance of apple-green birefringence.
The Screening test result performed in
Congo Red agar media revealed a glimpse of the comparative account of cellulolytic potential of the three strains involved and is given in Table 3.
Petriplates containing BHI medium with
Congo red dye, supplemented with 20% glucose and without glucose were prepared and the S.
Results: Of 81 isolates, biofilm production was detected in 43% isolates using
Congo red method while microtiter plate assay showed biofilm production in 92% isolates.
The results showed that the synthesized nanoparticles have adsorption properties of
Congo Red (CR).
In addition, biopsy of an asymptomatic organ or tissue, such as the liver or a less-invasive and highly effective needle aspiration biopsy of subcutaneous adipose tissue, can be stained with
Congo red to determine the presence of systemic amyloidosis.
The diagnosis of AL amyloidosis is made by staining a bioptic sample of skin or another affected organ tissue with
Congo red dye and viewing it under a polarizing microscope.
Figure 3a showed that amyloid granules were visible below the epithelium in the gastric antrum and figure 3b showed that amyloid granules were stained positive on
Congo red staining in the gastric antrum.
Bone marrow biopsy revealed 5% atypical plasma cells with lambda restriction,
Congo red positive amyloid deposits, and mass spectrometry consistent with AL (lambda)type amyloidosis (Figure 3).
The histopathological picture (
Congo red staining) is shown in Figure 3(b), with the high magnification figures of
Congo red staining (Figure 3(c)) and its green birefringence (Figure 3(d)).
Congo red (CR) (Aldrich-Sigma) was used as a model pollutant dye.
