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Caption: Figure 6:
Congo red staining in lung biopsy.
All types of amyloid protein stain with
Congo red and exhibit apple green birefringence on polarized microscopy [4].
(d) Deposits of amyloid material showing birefringence under polarized light (
Congo red stain) (x200).
where [C.sub.b] represents the amount of
Congo Red dye bound to aggregated peptide, [A.sub.541] is the optical absorption measured at the wavelength of 541 nm, [A.sub.403] is the optical absorption measured at the wavelength of 403 nm, 478000 is the extinction coefficient at 541 nm, and 38100 is the extinction coefficient at 403 nm.
All isolates absorbed little
Congo red to produce colonies that were pale pink to whitish in color.
cultivated on Luria Bertani agar plates supplemented with
Congo red express a phenotype known as "rdar" (red, dry and rough) morphotype [7].
Biofilm formation can be detected by Tissue Culture Plate Method (TCP), Tube Method (TM) and
Congo Red Agar (CRA) Method.
In 1924 Divery found that SPs consisted of amyloid using
Congo red staining.
The biopsy revealed amorphous material deposited in the vascular walls and apple-green birefringence in a polarization examination with
Congo red staining which proved amyloidosis.