Comparison between
Congo red agar test and PCR test results for icaA and icaD gene
Congo red agar, n (%) Total P-value Positive Negative Chi-square N=26 N=24 value icaA Positive 19 (73.1%) 10 (41.7%) 29 (58.0%) 0.025 (*) 5.1 Negative 7 (26.9%) 14 (58.3%) 21 (42.0%) icaD Positive 13 (50.0%) 17 (70.8%) 20 (40.0%) 0.133 2.3 Negative 13 (50.0%) 7 (29.2%) 30 (60.0%) df icaA Positive 1 Negative icaD Positive 1 Negative
Evaluation of modified
Congo red agar for detection of biofilm produced by clinical isolates of methicillin-resistance Staphylococcus aureus.
Herein, the PmPD/rGO/NFO composite shows highest adsorption capacity for
Congo Red (CR), methyl orange (MO), methyl blue (MB) and toxic heavy metal ion Cr(VI).
Congo red stain (20 x magnification) under unpolarized light (top) and polarized light (bottom) showing amyloid deposits (pink and amorphous) and positive green-apple birefringence under polarized light consistent with amyloidosis of the bladder.
Xiao et al., "Photocatalytic decolorization and degradation of
Congo red on innovative crosslinked chitosan/nano-CdS composite catalyst under visible light irradiation," Journal of Hazardous Materials, vol.
In lung biopsy, amyloid deposits were identified, which show green birefringence when stained with
Congo red and are visible under polarized light [14].
The pathologic findings (Figure 2) revealed the presence of amyloid proteins that appeared as homogeneous eosinophilic materials on hematoxylin-eosin and stained on
Congo red with apple green birefringence on polarized microscopy (data not shown).
The definitive diagnosis depends largely on histopathology confirmed by positive
Congo red staining.
The A[beta] fibrils and the oligomerization process [55-58] have been well characterized by multiple physicochemical measurements including transmission electron microscopy (TEM) [59], X-ray diffraction [60, 61], mass spectrometry (MS) [62-64], 2D infrared spectroscopy (2D-IR), circular dichroism (CD),
Congo Red dye binding (CR), Thioflavin-T fluorescence (TTf) [65, 66], SDS-gel electrophoresis (SDS-PAGE) [67], atomic force microscopy (AFM) [68, 69], gel filtration [70, 71], fluorescence resonance energy transfer (FRET) [72, 73], dynamic light scattering (DLS) [74], Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance imaging (NMR) [75].
Gram Staining and Ability to Absorb
Congo Red. Gram staining and microscopy were carried out to determine if the cultures were Gram negative or positive.