coculture

To challenge our coculture model, we additionally included (4) prohaptens (3-aminophenol, resorcinol and acetaminophen) that remained negative with regard to the induction of keratinocyte responses even after exposure in the presence of additional S9 mix (modified KeratinoSens (Natsch and Haupt, 2013)).

Cell flasks were incubated 5 hours prior to flow cytometry analysis and cells in plates were incubated 24 hours for coculture experiments.

Therefore, to mimic in vivo conditions, strains were inoculated individually or together (coculture) at [10.sup.6] colony forming units (CFU) for PAO1 and [10.sup.2] CFU for VPI 4355.

Furthermore, in the in vivo environment, these cells do not contact directly, so their coculture may influence and distort the results.

Schmal, "The trans-well coculture of human synovial mesenchymal stem cells with chondrocytes leads to self-organization, chondrogenic differentiation, and secretion of TGF[beta]," Stem Cell Research & Therapy, vol.

in potting soil samples (14-16), and the use of amebal coculture techniques has shown promising results in recovering L.

A coculture system comprising human intestinal epitheliallike Caco-2 cells and phorbol myristate acetate (PMA)-stimulated THP-1 macrophage cells was used.

Final density of splenocytes was adjusted to 2.5 x [10.sup.5] cells per well for coculture with AT-MSCs.