Nuclear translocation of
DNase II and acid phosphatase during radiation-induced apoptosis in HL60 cells.
In order to eliminate contaminating protein and nucleic acids, treatment with proteinase K,
DNase and RNase was performed prior to extraction step.
Total RNA was prepared using TRIZOL reagent (Invitrogen) and treated with
DNase I (Invitrogen) according to the manufacturer's instructions.
* Treatment: We put Lauren on albuterol and recombinant human
DNase respiratory treatments, pancreatic enzymes, and multivitamins with extra lipid-soluble vitamins and calcium supplements.
RNA cleanup was performed if samples were contaminated with DNA by treating the RNA preparation with an additional
DNase I digestion and, if required, a final cleanup using the RNeasy Mini Kit (Qiagen).
The
DNase inhibitor activity measured in the absence of guanidine- HCl represents the amount of G- actin in a sample and the activity after treating the sample with guanidine-HCl measures total actin.
Bacterial amylase can be purchased (Carolina 202360) and has little to no
DNAse activity.
Second, even after heating the slurry made from M, domestica to 80 [degrees]C for 20 min, which was intended to eliminate the
DNase activity, plasmid DNA degradation still occurred.
And less than two-thirds of patients who would benefit from
DNase are receiving it.
Components Volume 5 x RT PCR Buffer (Invitrogen) 3 [micro]L RNase
DNase free distilled water 2.83 [micro]L 0.1 M DTT (Invitrogen) 1 [micro]L DNTP (Invitrogen) (10 mM) 1 [micro]L RNase OUT (Invitrogen) 0.67 [micro]L Superscript II RT (Invitrogen) 1 [micro]L Reverse Primer (25 pM) 0.5 [micro]L DNase-treated RNA sample 10 [micro]L Total 20 [micro]L TABLE 2.
To test whether it is
DNase that helps strep A bacteria escape NETs, the researchers created a mutant strain of the bacteria that lacked the gene that encodes for
DNase.
RNA samples were treated with
DNase I at 37[degrees]C for 30 min to remove genomic DNA.