The present goal of the study was to characterize the hemoglobin of Leaf-nosed viper (Eristicophis macmahonii), member of family Viperidae.
Here we describe the preliminary studies on hemoglobin of Leaf-nosed viper.
Blood Sample Collection and Hemolysate Preparation: Leaf-nosed viper (Eristicophis macmahonii) specimens were collected from Nushki, Balochistan, Pakistan.
Purification of Hemoglobin: Adult Leaf-nosed viper (E.
[alpha]A globin chain: The N-terminal amino acid sequence of intact [alpha]A globin chains of Leaf-nosed viper (E.
[beta]I globin chain: The [beta]I globin chain sequence of Leaf-nosed viper showed highest similarity (93.3%) with [beta]I chain of Texas indigo snake (Drymarchon melanurus erebennus).
[beta]II globin chain: The [beta]II globin chain of Leaf-nosed viper showed maximum sequence similarity (93.3%) with [beta] chain sequence from Indian cobra (Naja naja) and Blue-lipped sea krait (Laticauda laticaudata).
[alpha]A globin chain: Multiple sequence alignment of the of [alpha]A globin chain of Leaf-nosed viper is shown in Fig.
[beta]I globin chain: Multiple sequence alignment of [beta]I globin chain of Leaf-nosed viper is shown in Fig.
In contrast to [alpha] chain, the [beta] chain of Leaf-nosed viper has highest similarity 93.3% with Texas indigo snake and least similarity 70% with Indian cobra.
Most bats are insect feeders, while the New World Leaf-Nosed bats eat nectar, fruit, frogs, lizards and even blood.
They found that the emergence of a new skull shape in New World Leaf-Nosed bats about 15 million years ago led to an explosion of many new bat species.
In the vicinity of the area chosen for the airport site by the government, there are apparently three limestone caves and research conducted in September revealed 350 bats, including 100 old world
leaf-nosed bats according to Kyodo News.
