Two staining methods were used: Panotico Rapido, and
Giemsa.
The slide was then dipped in 10%
Giemsa's stain for 10 min.
Neutrophils for the Wrights
Giemsa stain (A), Papanicolaou (B), Toluidine blue (C) and Methylene blue (D).
These inclusions were then visible in the
Giemsa stained film that was prepared in the laboratory.
Diagnoses were made by pathologists, cytotechnologists, or laboratories from slides stained by either the modified
Giemsa or Pap stain method.
These staining techniques are beneficial as they are easy to do and have high sensitivity and specificity.6 For rapid identification of fungal pathogens, conventional techniques include 10% KOH and lacto phenol cotton blue wet mounts.8 Gram and
Giemsa staining techniques are used for identification of bacteria6 and Modified Ziehl-Neelsen staining for Nocardia identification.9 These staining techniques provide a preliminary diagnosis, when culture results are pending.10
Los amastigotes pueden ser identificados por tincion con Wright o
Giemsa en preparaciones como frotis o secciones de tejidos obtenidos de lesiones en piel, bazo, higado y medula osea, o bien aspirados de nodulos linfaticos.
Purple coloured intracytoplasmic inclusions in epithelial cells were confirmative with
Giemsa stain.
Hastadan alinan kan orneginin
Giemsa boyasi ile hazirlanan kalin damla ve ince yayma kan preparatlarinda cok sayida (>%5) P.